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The effects of cultural conditions in the rice straw media for cellulolytic enzymes production by Pleurotus sajor-caju were investigated. The optimum moisture content, pH and temperature for enzymes production were 60%, 7.0 and 35℃ in C₁-cellulase, and 60%, 5.0 and 25℃ in C₁-cellulase, and 60%, 7.0 and 20℃ in β-glucosidase, respectively. When light was irradiated during the cultivation period, C₁-cellulase and β-glucosidase production were decreased but C₁-cellulase production increased at 500∼1,000 lux. During the cultivation period, C₁-cellulase production was contrary to C_x-cellulase and β-glucosidase. Among the various materials added, rice bran was effective to C₁-cellulase production, cotton seed cake and rice bran to C_x-cellulase production, and defatted soybean and fish meal to β-glucosidase production. The optimum concentration of rice bran for enzymes production were 20% in C₁-, C_x-cellulase and 10% in β-glucosidase.
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